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Molecular Characterization of hsp20, Encoding a Small Heat Shock Protein of Bifidobacterium breve UCC2003▿ †

机译:hsp20的分子特性,编码短双歧杆菌UCC2003的小热激蛋白

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摘要

Small heat shock proteins (sHSPs) are members of a diverse family of stress proteins that are important in cells to protect proteins under stressful conditions. Genome analysis of Bifidobacterium breve UCC2003 revealed a single sHSP-encoding gene, which was classified as a hsp20 gene by comparative analyses. Genomic surveillance of available genome sequences indicated that hsp20 homologs are not widely distributed in bacteria. In members of the genus Bifidobacterium, this gene appears to be present in only 7 of the 30 currently described species. Moreover, phylogenetic analysis using all available bacterial and eukaryotic sHSP sequences revealed a close relationship between bifidobacterial HSP20 and the class B sHSPs found in members of the division Firmicutes. The results of this comparative analysis and variation in codon usage content suggest that hsp20 was acquired by certain bifidobacteria through horizontal gene transfer. Analysis by slot blot, Northern blot, and primer extension experiments showed that transcription of hsp20 is strongly induced in response to severe heat shock regimens and by osmotic shock.
机译:小型热激蛋白(sHSP)是多种应激蛋白家族的成员,这些家族对于细胞在应激条件下保护蛋白很重要。短双歧杆菌UCC2003的基因组分析显示单个sHSP编码基因,通过比较分析将其归类为hsp20基因。对可用基因组序列的基因组监测表明,hsp20同源物并未在细菌中广泛分布。在双歧杆菌属的成员中,该基因似乎仅存在于目前描述的30个物种中的7个。此外,系统发育分析使用所有可用的细菌和真核sHSP序列揭示了双歧杆菌HSP20与在Firmicutes部门的成员中发现的B类sHSP之间的密切关系。该比较分析的结果和密码子使用含量的变化表明,hsp20是由某些双歧杆菌通过水平基因转移获得的。通过槽印迹,RNA印迹和引物延伸实验的分析表明,响应于严重的热休克方案和渗透压休克强烈诱导了hsp20的转录。

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